spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Characterization of the MBD1 allele. (A) Overview indicating the exon-intron structure of the MBD2/3 locus and the position of the P element insertion. (B) Northern analysis of embryonic poly(A)+ RNA from 0- to 6-hour-old embryos. The upper panel shows significant levels of MBD2/3 mRNA in control (yw) embryos, while no signal was detectable in MBD1 mutant embryos. A probe against the elongation factor EF1{alpha}48D was used as a loading control (lower panel). (C) Western analysis of 0-6 hours-old embryonic protein extracts. Nucleosome assembly protein 1 (NAP1) was used as loading control. (D) Confocal sections from embryos double immunostained with antibodies against MBD2/3 (green) and DNA (red). Mutant embryos showed only background levels for MBD2/3, while the DNA signal showed no quantitative differences between mutant and control.





Right arrow Return to article