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Fig. 3. Functional interaction between MBD2/3 and MI-2. (A) MBD1/MI-24 compound heterozygotes (left panel) showed significantly smaller and rougher eyes than either parent strain (middle and right panels). (B,C) Analysis of MI-2 protein distribution in control (B) and mutant (C) embryos by double immunostaining with antibodies against MBD2/3 (green) and DNA (red) and subsequent confocal microscopy. Scale bars: 5 µm. (B) MI-2 was ubiquitously distributed in wild type nuclei. (C) MI-2 was displaced from a subset of chromosomal sites in MBD1 embryos.





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