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Fig. 5. MBD2/3 interacts with CpT/A-methylated oligonucleotides in band shift assays. Drosophila GST-MBD2/3, GST-MBD2/3{Delta} and human GST-MBD2 were analyzed for their ability to bind to radioactively labelled oligonucleotide probes. Binding assays were performed either in the absence (-) or presence (+) of recombinant proteins and competed with a 400-fold excess of the corresponding unlabelled probe (c). (A) Binding assays with a CpG-methylated probe (m) or with the corresponding unmethylated probe (u). (B) Binding assays with a CpT/A-methylated probe (m) or with the corresponding unmethylated probe (u). Arrowheads indicate the position of shifted oligonucleotide probes.





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