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Fig. 6. The localization of MBD2/3 depends on DNA methylation. (A) Embryos were demethylated with the DNA methyltransferase inhibitor 5-azacytidine and double immunostained with antibodies against MBD2/3 (green) and DNA (red). This caused a readily detectable displacement of MBD2/3 from DNA. (B) For comparison, pictures from mock-treated embryos are also shown. Scale bars: 25 µm, insets show fourfold magnified details.





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