|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Phenotype of brk point mutants and CtBP and gro
mutants. Third instar wing discs containing mutant clones, marked by the loss
of a ubiquitous GFP transgene, and stained for omb (lacZ,
anti-ßgal), Sal (antibody) and/or vg-QE (lacZ, anti-ßgal)
expression. (A,B) brkF124 clones show ectopic expression
of Sal and omb within the wing pouch (arrow), and expansion of the
vg-QE domain. (C) brkF138 clones. (D) Detail from boxed
area in C, showing a large clone in the posterior compartment. The edge of the
normal Sal and omb domains are shown in red and blue, respectively,
in part i, and in white in parts ii and iii (these are approximations). If
this were a null mutant clone, Sal and omb would be expressed in all
of the mutant cells in the wing pouch, and omb would extend outside
of the pouch (see A). However, there is no ectopic omb expression in
brkF138 mutant cells, apart from possibly an expansion to
one or two cells wider than normal (note, omb is on the same
chromosome as brk, so that omb expression is upregulated in
brk mutant cells within its endogenous domain because these cells are
now homozygous for the omb enhancer trap). There is some ectopic Sal
expression, but only in mutant cells within the endogenous omb domain
(arrow in part ii) and not more laterally. (E) vg-QE expression is expanded
laterally in some brkF138 clones (arrow). (F-H) CtBP
gro double mutant clones are similar to those of
brkF138, only showing ectopic Sal expression (F, arrow)
immediately adjacent to the endogenous domain (when located in the
omb domain, not shown). By contrast, Sal is not ectopically expressed
in any CtBP single mutant clones (G), whereas there is an occasional, minor
deregulation of Sal in gro clones (H, arrow).
|
