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Fig. 4. Complementation of Arabidopsis B-class mutants ap3 and
pi by their maize orthologs. (A-E) Arabidopsis flowers of
(A) wild type, (B) ap3-3 mutant, (C) ap3-3 with
AP3pro:Si1 transgene, (D) pi-1 mutant with
AP3pro:Zmm16 transgene (note white sepal margins), and (E)
ap3-3 pi-1 double mutant with both AP3pro:Si1 and
AP3pro:Zmm16 transgenes (note white petals and sepals).
(F-J) Scanning electron microscopy (SEM) of flowers from the same plants from
which the flowers shown in A-E were obtained, in the same order. (K-O) SEM of
abaxial petal or second whorl organ epidermis from flowers shown in F-J, in
the same order. Cells of wild-type petal epidermis (K) are rounded and no
guard cells are present. Cells of second whorl `sepal' epidermis of
ap3-3 mutant (L) are elongated and irregular, with many guard cells.
Rescued mutants (M-O) have epidermal cells that are intermediate in shape
between those seen in K and L, with occasional guard cells. Note the lack of
elongated epidermal cells characteristic of sepals. Arrows in L, N and O
indicate guard cells. Scale bars: 0.5 mm in F-J; 50 µm in K-O.
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