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Fig. 2. Anterior dMP2 neurons survive when apoptosis is prevented. (A) Wild-type expression of dMP2-GAL4/+;UAS-nls-myc-EGFP/+ in late embryos (air-filled trachea stage). Only the three posterior pairs of dMP2 neurons are apparent. (B) In late embryos homozygous for the H99 deficiency, which removes hid, grim and rpr, anterior dMP2s survive. (C) Anterior dMP2s also survive in XR38/H99 mutant embryos (dMP2-GAL4,H99/XR38; UAS-nls-myc-EGFP), indicating that rpr mediates dMP2 death. (D) Postmitotic expression of anti-apoptotic baculoviral P35 in the dMP2 neurons (UAS-p35/+; dMP2-GAL4/dMP2-GAL4, UAS-nls-myc-EGFP) efficiently suppresses cell death. Surviving anterior dMP2s always express Odd in both XR38/H99 embryos (E) and when apoptosis is blocked with UAS-p35 (F). For each genotype in A to D n>10 VNCs (see Fig. 7B for additional numbers). (G,H) In larvae, anterior dMP2s express Proctolin in 30% and 21% of cases, respectively (n>37 rescued cells). (I) rpr^GAL4 expression in a stage 16 embryo (rpr^GAL4/+; UAS-nls-myc-EGFP). Expression is dynamic, but double labeling with Odd (J) reveals that rpr^GAL4 expression is often observed in 45% of anterior dMP2 neurons. It is, however, always absent from posterior dMP2 neurons (n>40 VNCs).

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