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Fig. 2. EDEN-BP morpholinos affect somite segmentation in Xenopus embryos.
(A) Xenopus embryos were left uninjected (lane 1), or were injected
at the two-cell stage into both blastomeres with a mixture of 25 ng of each
EDEN-BP morpholino, in the absence (lane 2) or presence of (2 fmol, lane 3;
0.5 fmol, lane 4) EDEN-BP mRNA. Total protein extracts from embryos collected
at stage 25 (tailbud) were analyzed by western blotting using an anti-EDEN-BP
antiserum (upper panel) and an anti-cdc2 A17 monoclonal antibody (lower
panel). (B-G') Embryos were injected into one blastomere at the
two-cells stage with 50 ng of control morpholinos (C-Mo), 25 ng of each
EDEN-BP-Mo (E-Mo), or 25 ng of each EDEN-BP-Mo and 2 fmol of EDEN-BP mRNA
(E-Mo+R). They were allowed to develop until stage 26 and were then processed
for immunohistochemistry with the myotome-specific 12/101 monoclonal antibody
(B-D'), or for in situ hybridization with a MHC4 probe (E-G').
Only photographs of the injected sides are shown. B'-G' are higher
magnifications of B-G, respectively. Asterisks highlight successive somites
for embryos showing segmentation.
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