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Fig. 3. Analysis of embryos injected with anti-EDEN-BP antibodies. (A-D) Embryos
were injected into one blastomere at the two-cell stage with control
(non-immune, C-Ab) or anti-EDEN-BP (E-Ab) antibodies. They were allowed to
develop until stage 39 and then were stained with the myotome-specific 12/101
monoclonal antibody. C and D are higher magnifications of the lower embryos in
A and B, respectively. (A,C) Injected sides and (B,D) non-injected sides of
the same embryos. (E-G) Hematoxylin and Eosin-stained horizontal sections of
stage 39 embryos injected at the two-cell stage with 200 ng of anti-EDEN-BP
antibodies. (F,G) Higher magnifications of the somites of the non-injected (F)
and injected (G) sides. n, notochord; s, somite. Arrow indicates the injected
side. Asterisks highlight successive somites for embryos showing segmentation.
Scale bars: 50 µm.
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