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Fig. 4. Dorsal-to-ventral migration within the basal telencephalon. (A) A grafting
experiment performed at E13.5, in which ventrally migrating cells are
analysed. The box delineates the area shown in C-E''. (B) Example of a
graft in which GFP-positive cells have reached the POa. Arrows in C show
co-localization of COUP-TFI (red) with GFP (green), whereas no co-labelling
with COUP-TFII (red) has been identified (D) within the migratory cells.
(E-E'') Arrows in (E'') indicate that migrating cells are neurons by
double labelling of GFP (green) with Tuj1 (red). (E'') is a merge of
panels (E) and (E'). (F) Double immunofluorescence of COUP-TFI (green)
and GABA (red) shows that some COUP-TFI-positive cells (arrows) are GABAergic
in the ventral migratory stream. (G) By electroporation of a GFP-expressing
construct into the MLGE region, GFP-positive cells migrate ventrally, and
these cells express COUP-TFI (arrows in H). Similarly, by inserting a DiI
crystal into the MLGE region, fluorescent cells are detected ventrally, in the
POa/hypothalamic region (I). These cells co-express COUP-TFI (J). The arrow
indicates a leading process. Note that the diffuse red signal in J is the
result of the DiI signal after fixation.
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