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Fig. 6. COUP-TFI modulates dorsal and ventral migration in brain slices. (A) Summary of the type of experiments performed on E13.5 mouse brain slices. On one side, a GFP-expressing vector is injected as a control, while on the contralateral side an injection of a COUP-TFI-IRES-GFP expressing vector shows an increased rate of migrating cells. (B) The average value of the GFP/GFP_tot ratio (black columns) or the GFP-COUP-TFI/GFP_tot ratio (white columns) of all of the injected slices, calculated independently in the cortex or ventrally in the POa (see also Materials and methods). Bars indicate standard errors of the mean. In the COUP-TFI injected side, 72±4% of the GFP-positive cells migrate into the cortex, versus 24±4% of the GFP-positive cells in the control side. For the POa ectopic expression of COUP-TFI, 46±3% of the GFP-positive cells were induced to migrate, versus 17±3% in the control situation. Significant P-values are indicated (for the cortex P=0.0008, for the POa P=0.004). Example of a control (C) and a COUP-TFI overexpressing (D) brain slice after 60 hours of incubation. The vectors were focally injected into the MLGE region. Note that in the control situation (C), a few cells were found in the cortex (C'), while the majority remain compacted in the injection point. (D) After injection of high levels of COUP-TFI into the MLGE region, the GFP-positive cells are more dispersed (arrowheads) around the injection point, and a more pronounced contingent of cells migrates in the dorsal and ventral directions. MZ, marginal zone; Cx, cortex.

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