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Fig. 1. Typical confocal sections through a chick embryo show the dense streams of
cranial neural crest cells and various features of cell shapes and extensions
within the migratory streams. (A) This typical embryo was co-injected with
Gap43-tagged EGFP (green) and MRFP (red) to label the cell plasma membranes
and nuclei, respectively, and re-incubated for 15 hours. Neural crest cells
are seen along the migratory routes in dense streams emanating from r4 and r6,
and individual cells leaving from r7. Many of the cells have nearly reached
the destination branchial arches (ba2-ba4). The individual boxes highlight
regions that are magnified in the remainder of the figure. (B) Cells within
the r4 stream are relatively densely packed. Some of the individual
Gap43-labeled cells reveal filopodial extensions, which overlap with
neighboring MRFP-labeled cells. The Gap43-labeled cells also show the
difference in potential extent to which an individual cell has contacts with
other neural crest cells in the stream. (C) Interestingly, some neural crest
cells from r5 travel laterally to the edge of r5 and then move in the
anteroposterior direction toward a neighboring migratory stream along the edge
of the otic vesicle. (D) Neural crest cells that have nearly reached the
branchial arch destinations are very closely packed within the migratory
stream. The protruding extensions of the cells appear to stretch out from the
cell body such that an individual cell may contact a cell that is not within
its local neighborhood. (E) A closer look at individual cells within the
stream reveals that an individual cell may extend many thin filopodial
protrusions. Scale bars: 50 µm in D; 10 µm in E. ov, otic vesicle.
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