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Fig. 5. Neural crest cell-cell contact via filopodia may alter the trajectory of a
trailing cell. Selected images from a typical time-lapse imaging session show
neural crest cells within the r6 stream. The direction of migration is to the
right. Three individual neural crest cells are highlighted (green, red and
blue), with the blue cell and the green cell positioned downstream (distal) to
the red cell. First, the trailing cell (red) senses the local environment with
short filopodial extensions (t=0). The downstream cell ahead in the r6 stream
(blue) is migrating toward the branchial arch. The red cell extends a
filopodium in the direction of the blue cell (t=1 hour). The blue-colored cell
moves slightly backward. Then, the filopodium from the red cell makes contact
with the blue cell, and elicits a response from the blue cell in the form of a
short filopodial extension in the reverse direction of travel (t=1 hour 5
minutes). Both the red and blue cells retract their filopodia (t=1 hour 30
minutes). The red cell then begins to migrate in the direction of the point of
contact with the blue cell (t=3 hours) and migrates further downstream to
catch up with the blue cell. The images in the left column have been embossed
from the raw data in Adobe Photoshop 7.0 to bring out the edges of the cells.
The images in the right-hand column are tracings over the cells from the raw
data. The + sign (t=3 hours) marks the original location of the red cell
before contacting and migrating toward the blue cell. Scale bar: 30 µm.
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