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Fig. 6. Neural crest cells within a stream may be connected by long, thin cellular
processes. In a typical cell migration stream, many neural crest cells appear
to be connected by long, thin cellular processes. (A) Two neural crest cells
appear to be linked by a long, thin cellular process (arrow) that stretches
between two cells and intertwines around a cell. A fragmented cellular process
lies adjacent to one of the cells (arrowhead). This image represents the
projection of 30 confocal slices at 1 µm intervals. (B) A 3D rendering of
the confocal z-stack reveals the long, thin cellular link (arrow) and
short-range contacts. The fragments of a broken cellular process are also
shown (arrowhead). (C) A close-up of the 3D rendering of the z-stack
and rotation reveals the actual physical connection of the cellular process
between the two cells (arrow). The z-stack has been turned by
approximately 30 degrees around the y-axis. The embryo was injected
with Gap43-EGFP (green) and H2B-MRFP (red) into the neural tube,
electroporated and re-incubated for 12 hours. Scale bar: 30 µm.
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