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Fig. 4. Kit⁺ cells in and around developing sensory ganglia co-localise with blood vessels. (A) Section through a dorsal root ganglion in an E10.5 W^lacZ/+ embryo, stained for CD34. (B) Section of an E10.5 W^lacZ/+ embryo stained for F4/80 in an area just adjacent to ganglion. (C) Same region as is shown in B, stained for ßgal. (D) Merged views of B and C. (E) View of the dorsolateral region of an E10.5 W^lacZ/+ embryo stained for CD34 + F4/80 (red), revealing the blood vessel network, and ßgal (green). Bright ßgal⁺ cells are seen under the ectoderm (closed arrow), as well as pale ßgal⁺ cells associated with blood vessels (double-headed arrow). Some ßgal⁺ cells are also present within the NT. Dotted lines indicate either the surface of the embryo, the location of the ganglion, or the border of the NT. bv, blood vessel; drg, dorsal root ganglion. Scale bars: 50 µm in A-D; 150 µm in E.

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