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Fig. 6. Pancreatic defects in E17.5 Smad2 mutant embryos. (A,E) Ngn3 expression (blue) detected by antisense RNA in-situ hybridization. (B,F) NKX2.2 expression (brown nuclei) adjacent to ductal epithelium. (C,G) NKX6.1 expression (brown nuclei marked by arrows). (D,H) Insulin expression (green) detected by IHC and confocal microscopy. Inset, H: NGN3+ nuclei (red) adjacent to insulin+ cells (green). Images are representative of four or more animals per genotype.





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