DEV01542 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. A three-dimensional dataset of a late gastrula frog embryo, presented from several perspectives.

• Movie 2 -

  Movie 2. A movie of normal frog gastrulation, viewed from the vegetal side.  Note the coordinate inward movement of yolk plug and surface cells, with simultaneous blastopore closure. See also Fig. 5A,a¢.

• Movie 3 -

  Movie 3. Gastrulation in an embryo circumferentially injected with Xdd1. Note the inward movement of yolk plug and surface cells without significant blastopore closure. See also Fig. 5B,b¢.

• Movie 4 -

  Movie 4. Normal blastopore closure in a control embryo. See Fig. 7A.

• Movie 5 -

  Movie 5. Impaired blastopore closure in an embryo injected ventrally with Xdd1. See Fig. 7B.

• Movie 6 -

  Movie 6. Severely impaired blastopore closure in an embryo dorsally injected with Xdd1.  See Fig. 7C.

• Movie 7 -

  Movie 7. Severely impaired blastopore closure in an embryo circumferentially injected with Xdd1. See Fig. 7D.

• Movie 8 -

  Movie 8. A three dimensional dataset of a late gastrula frog embryo, depicting the surface of the embryo in yellow, and the internal cavities in pink. The blastocoel (large cavity on the left) is connected to the archenteron (large cavity on the right) by a series of smaller channels and cavities.