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Fig. 2. Metrics for quantifying gastrulation. (A) Blastopore closure: ratio of the
blastoporal surface area (yellow) to the surface area of the vegetal
hemisphere (green) of the embryo. (B) Convergent extension: length-to-width
ratio of the Xnot domain (red outline). (C) Spherical approximation to the
embryonic surface visualized by reprojecting the 2D image into 3D. White lines
in B and C indicate the dorsal midline. (D) Archenteron measurement: distance
in degrees between the dorsal lip of the blastopore (blue line) and the
anterior limit of the archenteron (white line); degrees of arc between the
anterior limit of the Xnot domain (pink line) and the anterior limit
of the archenteron (white line); degrees of arc between the dorsal
mesendodermal mantle (orange line) and the center of the blastopore. (E)
Archenteron inflation: area of the archenteron at the mid-sagittal plane
divided by the area of the embryo at that plane.
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