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Fig. 1. Expression of endothelial cell markers in the yolk sacs. (A) lacZ staining for endoglin, in Eng+/– mice with ß-gal knocked into the endoglin locus. (B-D) Immunohistochemistry for PECAM1 (B), Flk1 (C) and endoglin (D) in wild-type yolk sacs. Yolk sac was sectioned in plastic following staining as whole mounts in all cases. Scale bar: 0.5 mm. Abbreviations: EC, endothelial cell layer; end, endoderm; mes, mesothelial cell layer.





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