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Fig. 3. GalT I-null sperm are more sensitive to Ca2+ ionophore than
wild-type sperm during in vitro capacitation. Wild-type (gt +/+) and
GalT I-null (gt –/–) sperm were capacitated for varying
periods of time before incubation with 10 µM A23187 or an equal volume of
DMSO. GalT I-null sperm show higher basal levels of sensitivity to
ionophore-induced acrosome reactions, as well as greater sensitivity during
capacitation in vitro. Each point represents the mean±s.e.m.;
n=3 experiments. In a single experiment, the value of each data point
represents three determinations. Thus, each data point reflects data collected
from nine assays, each containing 200 sperm counted. *P<0.01.
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