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Fig. 7. GalT I-null sperm exhibit constitutively increased levels of cAMP during capacitation in HCO3– -containing or HCO3– -free media. Wild-type (gt +/+) and GalT I-null (gt –/–) were capacitated for varying periods of time in HCO3– -containing or HCO3– -free media. The cAMP in 1x106 sperm was extracted with ice-cold ethanol and detected by enzyme immunoassay. Each bar represents the mean±s.e.m.; n=4 experiments. For a single experiment, the value for each data point represents the average of two determinations (for a total of eight assays).





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