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Fig. 3. The ligand in the peripheral fraction of the ovulated zona pellucida (ZP)
is not a contaminant of the cortical granules. (A)
N-acetylglucosaminidase activity of fractions of the ovulated ZP
(three ZP equivalents/µl) prepared from eggs treated with 10 µM A23187
to induce the cortical reaction or DMSO, as control. (B) Biological activity
of the ovulated ZP fractions (three ZP equivalents/µl) prior to and after
treatment of the eggs with 10 µM A23187. Each bar represents the
mean±s.d.; n=2 experiments. In a single experiment, the value
for each data point represents the average of three determinations (for a
total of six assays per data point). The peripheral fraction contains variable
amounts of GlcNAc'ase, but this contribution does not account for its
biological activity.
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