|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Gene expression profiles in the dmtA– mutant. (A)
Developmental time course of gene expression. The ecmA and
ecmB (prestalk), cotB (prespore) and cprD
(growth/differentiation transition) markers are expressed with comparable
levels and timing in dimA– and wild-type cells, from
0-24 hours. The 
5 kb dimA transcript is also developmentally
regulated in wild-type cells but is absent in the mutant. However, a larger
transcript (>7 kb), due to a readthrough transcription into the blasticidin
resistance cassette, is detectable. Methylene Blue staining of ribosomal RNA
(rRNA) is shown as a loading control. (B) dimA transcripts are
expressed in both prespore and prestalk cells at the slug stage of
development, although the highest levels are detectable in prespore cells. The
purity of the isolated cell populations is indicated by the great enrichment
of ecmA and ecmB in prestalk cells, and cotB in
prespore cells.
|
