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Fig. 6. Measurement of DIF biosynthesis in dimA^–, and DIF response in dmtA^–. (A) dimA produces normal levels of cell-associated DIF-1. Cells were developed for the indicated times (hours) on agar containing ³⁶Cl^– and labeled compounds extracted with organic solvents, resolved by TLC and detected using a phosphorimager. The inclusion of the P450 inhibitor ancymidol (ancy) in the agar at the time points indicated results in the build-up of the DIF-1 breakdown product DIF-3. LCCs are late chlorinated compounds produced by stalk cells. (B,C) Stalk cell induction and spore cell repression in dmtA^–. The efficiency of stalk cell induction (B) or spore cell repression (C) by DIF-1 was measured in 8-Br-cAMP monolayers. No difference was observed between dmtA^– and wild type. Results shown are from a representative experiment. The experiment was performed at least three times.

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