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Fig. 1. Zebrafish Has genes and their expression during embryogenesis. (A) Homology tree of Has1, Has2, and Has3 family members based on amino acid sequences. The GenBank accession numbers for the sequences listed above are: Xenopus laevis xHas1 (X52958), Xenopus laevis xHasrs (AF015780), mouse mHas3 (MMU86408), human hHAS2 (HSU54804), mouse mHas2 (MMU52524), human hHAS1 (HSU59269), mouse mHas1 (D82964). (B) RT-PCR analysis of zebrafish has1, has2 and has3 expression during different stages of development. (C-J) Wild-type embryos stained for has2 mRNA (blue), and No tail (Ntl) protein (brown; F-J). (C) Early gastrulation (shield stage), animal pole view. Arrowheads indicate the dorsal shield devoid of staining. (D) Midgastrulation (80% epiboly stage), dorsal view. (E) 90% epiboly stage, lateral view, dorsal to the right. (F) End of gastrulation (100% epiboly stage), dorsal view on flat-mounted embryo; anterior to the left. (G-J) 8-somite stage: (G) dorsal view on flat-mounted embryo, anterior to the left; (H-J) optical cross sections at levels indicated in G.





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