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Fig. 3. has2 morphants are elongated, similar to Bmp mutants, but are not
dorsalized. (A-D) Live embryos at tailbud stage, lateral view, dorsal to the
right. (A) Wild type; (B) has2 morphant; (C) bmp2b/swr
mutant; (D) embryo co-injected with has2 MO and has2 mRNA.
(E,F) sox17 expression at tailbud stage, lateral views, dorsal to the
right. (E) Wild type; (F) has2 morphant. Arrow (E) indicates
forerunner cells; arrowhead (F) endodermal cells that have remained on the
ventral side. (G-I) pax2.1 expression at the 4-somite stage, lateral
view. Arrow (H) indicates midbrain-hindbrain boundary; arrowheads (G,H)
indicate presumptive pronephric duct cells. (G) Wild type; (H) has2
morphant; (I) swr mutant. (J-L) pax2.1 (marked as `p'),
myoD (`m'), hgg1 (`h') expression at the 10-somite stage;
dorsal views on regular (K) or flat-mounted (J,L) embryos. (J) Wild type; (K)
has2 morphant; (L) kny/glypican6 mutant. In J,
midbrain-hindbrain boundary is marked by an arrow, pronephric ducts by
arrowheads. (M,N) gata1 (`g') and myoD (`m') expression at
the 10-somite stage; dorsal view on flat-mounted embryos. (M) Wild type; (N)
has2 morphant. (O,P) krox20 (`k') and myoD (`m')
expression at the 10-somite stage; dorsal view on flat-mounted embryos. (O)
Wild type; (P) has2 morphant.
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