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Fig. 4. Loss of Has2 function leads to blockage of dorsal convergence, but not of
extension, and can be compensated by constitutively active Rac1. (A-I)
Distribution of labeled cell clusters at onset of gastrulation,
mid-gastrulation and end of gastrulation; lateral views, dorsal to the right.
(A-C) Wild type; (D-F) has2 morphant; (G-I) embryo co-injected with
has2 MO and caRac1 RNA. (A,D,G) Shield (6 hpf), directly
after labeling of cell clusters by fluorochrome uncaging; (B,E,H) 80% epiboly
(8 hpf); (C,F,I) tailbud (10.5 hpf). (J) Graph showing angle between labeled
cells and dorsal axis at different time points, measuring dorsal convergence
(indicated in B by blue bar). All clones start at 90° from the shield. (K)
Graph showing the anteroposterior extension of labeled lateral cell
populations (indicated in B by red bar) at different time points. In J and K,
10 embryos per treatment were evaluated, and standard deviations are
indicated.
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