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Fig. 6. Forced dorsal expression of has2 and constitutively active Rac1 lead to supernumerary lamellipodia, blocking axis extension. Extension movement of labeled populations of dorsal cells. (A-D) Wild-type control embryos; (E-H) embryos injected with has2 mRNA; (I-L) has2 morphant embryos; (M-P) embryos injected with caRac1 RNA; (R,S) embryos co-injected with has2 mRNA and dnRac1 mRNA. The first two columns show uncaging experiments. Lateral view of embryos at shield stage (6 hpf), directly after uncaging (A,E,I,M), or at tailbud stage (10.5 hpf; B,F,J,N). Columns 3 and 4 show morphology of clusters (C,G,K,O,R) or individual (D,H,L,P,S) dorsal cells labeled with membrane-localized GFP. Arrowhead (D) indicates single lamellipodium sometimes visible on narrow dorsal side of highly polarized axial cells of wild-type embryos. Arrowheads (H,P) indicate multiple lamellipodia in axial cells of has2 RNA- or caRac1 mRNA-injected embryos. (Q) Graph showing the amount of extension in the axis of wild-type, has2 MO-, has2 mRNA- or caRac1 mRNA-injected embryos. Per treatment, ten different embryos were evaluated, and standard deviations are indicated.

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