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Fig. 7. Has2 is required for migration of presumptive slow muscle and sclerotomal
cells during morphogenesis of somites, and for the migration of primordial
germ cells. (A-D) 30 hpf; smbpc in situ hybridization to mark slow
muscle cells of trunk somites: (A,C) wild type; (B,D) has2 morphant.
(A,B) Dorsal view, anterior left; (C,D) transverse sections. Arrows (B,D) mark
smbpc-positive cells located within the presumptive fast muscle
tissue. The lower side of the embryo in B is less affected, which might have
been the reason for its survival. The effects are restricted to the first 5-8
somites, consistent with previous findings indicating different properties
between such anterior and more posterior somites (see
van Eeden et al., 1996). (E,F)
twist in situ hybridization, at 24 hpf, to mark sclerotomal cells of
trunk somites. Arrowheads indicate dorsally migrated sclerotomal cells in wild
type (E), which remain ventral in has2 morphant embryos (F). (G,H)
Distribution of vasa-positive primordial germ cells, at 24 hpf, in
wild type (G) and has2 morphant (H). Arrowheads (H) point to ectopic
PGCs outside the genital ridges. hm, horizontal myoseptum; nc, notochord; nt,
neural tube; s, somite.
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