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Fig. 1. Generation of Six1-deficient mice. (A) Targeting strategy of
Six1. The Six1 gene consists of two exons (indicated by
boxes), and the coding regions are marked in black. The entire coding regions
were replaced with the EGFP gene (gfp) and the
hygromycin-B-phosphotransferase gene (hph). Open arrowheads indicate
the positions of PCR primers for genotyping. (B) Southern blot analyses of
wild-type (+/+), heterozygous (+/–), and homozygous (–/–)
mutant neonates. Tail DNA was digested with NcoI and hybridized to
5' probe (upper panel) and 3' probe (lower panel). The size of
each band is indicated on the left side. (C) In situ hybridization to
Six1 in E10.5 wild-type and homozygous embryos. Absence of
Six1 mRNA was confirmed in the Six1-deficient embryo. dt,
diphtheria toxin A gene; N, NcoI site. Scale bar: 1 mm.
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