QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Sens prevents Egfr pathway activation in the nucleus. UAS constructs were ubiquitously expressed in clones using flpout-GAL4. (A-A''') Co-misexpression of UAS-Egfr^act and UAS-lacZ posterior to the morphogenetic furrow (MF). Elav (red) is expressed in almost all cells within the clone (blue). Sens (green) is not detected within the clone. (B,C) Co-misexpression of UAS-Egfr^act and UAS-lacZ anterior to and within the MF. (B-B''') Elav (red) is expressed within and surrounding the clone (blue). Sens (green) is not expressed within the clone but is ectopically induced non autonomously. (C-C''') dpERK (red) and Sens (green) are expressed non-autonomously. Together, B and C are consistent with the presence of ectopic MFs surrounding areas of Egfr activation. (D-D''') Misexpression of UAS-Egfr^act anterior to the MF. pointed (pnt) transcription (pnt-lacZ, blue) occurs in most ectopic Elav-positive (red) cells. (E,F) Co-misexpression of UAS-Egfr^act and UAS-sens anterior to the MF. (E-E''') pnt transcription (pnt-lacZ, blue) does not occur and numbers of Elav-positive cells (red) are greatly reduced in the clone, which is marked by the Sens expressing cells (green). (F-F''') dpERK (red) is expressed autonomously at a high level within the clone, which is marked by Sens-expressing cells (green). Thus, sens is sufficient to block Egfr-induced pnt transcription, photoreceptor differentiation, and ectopic MF generation, but does not prevent dpERK induction.

Return to article