|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Wee1-depletion using antisense morpholino oligonucleotides (MO). (A)
MO-Wee1 was injected into both cells of a two-cell embryo. Uninjected and
MO-Wee1-injected embryos were collected at various stages and lysates examined
by immunoblot analysis using anti-Wee1 antibodies (* indicates a nonspecific
band). (B) Two-cell embryos were injected with MO-Control, MO-Wee1 or
co-injected with MO-Wee1 and WT Wee1 RNA (MO-Wee1+RNA). Embryos were collected
at stage 10 and lysates examined as in A. (C) Embryos injected as in B were
collected at stage 8, 10 or 12. Lysates were examined by immunoblot analysis
using anti-phospho-Cdc2 or anti-Cdc2 antibodies. (D) The mitotic nuclei of
injected stage 11 embryos were visualized by whole-mount immunostaining using
phospho-histone H3 (
PH3). Mitotic index (n=10-12 embryos):
MO-control, 8.9% (4739 nuclei); uninjected, 9.9% (1221 nuclei); MO-Wee1, 24.5%
(4734 nuclei); MO-Wee1+RNA, 11.4% (2736 nuclei).
|
