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Fig. 5. Upregulation of Wee1 activity by tyrosine autophosphorylation is required
for normal gastrulation. (A) Both cells of a two-cell embryo were injected
with RNAs encoding KD-Wee1, YYY/FFF-Wee1 or WT-Wee1. Anti-Flag immune
complexes isolated from lysates prepared at stages 5, 8, 9 and 10 were
examined by immunoblot analysis using anti-phosphotyrosine and anti-FLAG
antibodies. (B,C) Embryos injected with MO-Wee1 alone, MO-Wee1+RNA (WT),
MO-Wee1+RNA (KD) or MO-Wee1+RNA (YYY/FFF) were scored for gastrulation defects
at stage 11. Number of embryos examined: MO-Wee1 (n=17); MO-Wee1+WT
Wee1 (n=34); MO-Wee1+KD-Wee1 (n=42); MO-Wee1+YYY/FFF-Wee1
(n=35). Rescue RNAs were injected at 2 ng/embryo. Lysates from the
injected embryos were examined by immunoblot analysis using anti-Wee1
antibody.
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