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Fig. 5. Delayed patterning of ventricular activation sequences by gadolinium-injection at E7.5. (A) Isochronal map of control E8 heart with the apex-to-base activation pattern. (B) As in A but gadolinium-injected heart still exhibiting an immature lateral activation pattern. (C) Proportion of hearts exhibiting mature (white), intermediate (see text) (gray) and immature (black) activation patterns following no injection (control), buffer and gadolinium injection. Chi Square test between buffer- and gadolinium-injected groups gave a significant P value of 0.00012, while it found an insignificant P value of 0.521 between uninjected (control) and buffer-injected hearts. n, number of hearts examined. at, atrium; avj, atrioventricular junction; ec, endocardium; ivs, interventricular septum; lv, left ventricle; ot, outflow tract; rv, right ventricle; tb, trabeculum.





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