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Fig. 3. Cytochalasin D results in disorganized fibres that fail to form a coherent
actin cable. Confocal analysis of phalloidin-stained f-actin (green) and
propridium iodide (red) was used to assess the effects of cytochalasin D on
the organisation of the actin structures within the pouch endoderm (A-D)
compared with an untreated specimen (E), all at equivalent stages. (A) Side
view of an embryo that was treated by introducing cytochalasin D-soaked bead
into the pharyngeal cavity at stage 14– and incubated for a further 5
hours; already there is evidence of aberrant pouch morphology, where pouches
are contorted (1pp) or `relaxed' (2pp and 3pp). (B) High magnification of the
dorsal tip of a second pouch shows that actin fibres fail to form a
supracellular cable when treated with cytochalasin D; this embryo was treated
by introduction of a bead into the pharyngeal cavity and incubated for a
further 6 hours. (C,D) Mitotic cells (white arrows) are clearly evident within
the pouch endoderm of embryos treated with cytochalasin D, either via a bead
(C) or injection (D), as they are seen in the pouch endoderm of untreated
embryos (E). CD, cytochalasin D. Anterior is towards the left.
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