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Fig. 5. Hh movement is independent of dynamin-mediated endocytosis. All the wing discs were derived from third instar larvae. Anterior is towards left and dorsal is upwards in all the wing disc images (A-E'). In all discs, the shi^ts1 clones are marked by the absence of ß-gal staining (green) and outlined with broken lines. The AP boundaries are determined by Ci staining (not shown) and are marked by solid lines. (A-C) Hh staining in a wild-type wing disc (A) and a disc carrying a shi^ts1 clone in the A compartment (B,C). Hh protein appears to be graded membrane staining and is concentrated in large punctate particles (indicated by arrows) in three or four cell diameters abutting AP boundary in wild-type disc (A). These large punctate particles are absent inside the shi^ts1 clone and can only be observed at the boundary between mutant and wild-type cells (arrows in B). (B') The merged image stained with Hh and lacZ. (C) A basal optical section of the same disc shown in B where Hh accumulation along the cell membrane in the clone is more prominent. (D,D') Ptc staining in a disc carrying shi^ts1 clones at AP boundary. Ptc expression is expanded anteriorly within the lower larger clone. Noticeably, the wide-type cells anterior to the upper small clone still express Ptc, suggesting that shi^ts1 cells cannot block Hh movement. (E,E') Ci staining in a disc carrying shi^ts1 clones. Ci expression pattern is not significantly altered in the clones.

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