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Fig. 5. Motor nuclei and ganglia of cranial nerves and reticulospinal neurons, and
neuronal architecture of the hindbrain in prep1.1 morphants. (A-D) At
48 hpf, isl1 expression is not affected quantitatively in the motor
nuclei of the cranial nerves in prep1.1 morphants (B,D), but the
motor neurons of the facial nerve (nVII) have not migrated caudally as in
wild-type embryos (A,C). (E-H) Retrograde labelling with rhodamine-dextran of
3-day-old (E,F) and 5-day-old (G,H) embryos. In prep1.1 morphants,
only r4 cells are labelled; the projection of their axons (arrows) is similar
to that of Mauthner cells but their morphology is slightly different. (I,J)
RMO-44 antibody staining of reticulospinal neurons at 48 hpf. Only Mauthner
cells are present in prep1.1-MO injected embryos. (K,L) At 30 hpf,
the staining pattern of acetylated tubulin antiserum shows that the neuropils
and commissural tract fibres, (which are mostly out of focus in wild-type
embryos) are much reduced or absent in prep1.1 morphants. Embryos are
in dorsal (A,B,E-L) or lateral (C,D) views, with anterior to the left
(A-D,K,L) or the top (E-L). ctf, commissural tract fibres; llf, lateral
longitudinal fascicle; Mth, Mauthner cells; mlf, medial longitudinal fascicle;
np, neuropil; nVa and nVp, anterior and posterior clusters of the trigeminal
motor nucleus; nVII and nX, motor nuclei of the facial and vagus nerves,
respectively; r, rhombomere; wt, wild-type.
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