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Fig. 1. Fgf signalling pathway serves as a relay for the cell-nonautonomous
induction of oep by the Tar*/Nodal pathway. (A-Z) Flat mounts of
chimeric embryos at the shield stage showing the expression of tar, oep,
cyc, sqt, ntl, fgf3 and fgf8 in blue (the probe is indicated in
the bottom left of each panel). The progeny of transplanted cells expressing
Tar* is revealed by immunocytochemistry (red fluorescence staining the green
fluorescent protein, except in C,D,J,K where brown staining reveals nls-ß
galactosidase). The genotype of the recipient embryo is shown in the bottom
right of each panel. +SU, embryos treated with 10 µM SU5402 after
transplantation. In some experiments, the recipient embryo was injected with a
morpholino oligonucleotide directed against fgf8 (8MO). All views are
of the animal pole. For all panels except C,D,J,K, a Normarski image and an
epifluorescence image were overlayed.
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