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Fig. 5. WOX9 mRNA expression in mutant embryos. (A-F) In situ
hybridizations with a WOX9 antisense probe (brown staining).
Longitudinal sections of developing seeds. The demarcation line between the
apical and central domain of the early embryo is indicated (open arrow heads).
White asterisks indicate brown color of the seed coat independent of the
staining reaction. (A,B) bdl embryos. WOX9 mRNA is detected
in the hypophysis, but not in the central domain of double octant embryos
corresponding to the 8-cell stage wild type (A, compare to
Fig. 4K) and early heart stage
(B, compare to Fig. 4M,N) of
wild-type siblings. (C) mp embryo. WOX9 mRNA is detected in
the hypophysis, but not in the central domain of embryos corresponding to the
heart stage of wild-type siblings (compare to
Fig. 4M,N). (D) WOX9
expression in a gn embryo; the stage shown corresponds to the heart
stage of wild-type siblings (compare to
Fig. 4N). WOX9 mRNA is
detected in several epidermal cells throughout the embryo (arrow), but not in
the hypophysis. (E,F) WOX9 expression in wild-type embryos, with
abnormal cell division patterns. (E) A double octant embryo resembling that of
bdl. WOX9 mRNA is detected in the central domain. (F) An abnormal
embryo, corresponding to the late globular stage. Expression of WOX9
is roughly similar to that of normally developed wild-type embryos (compare to
Fig. 4M). ad, apical embryo
domain; cd1 and cd2, doubled central domain of double octant embryos; hy,
hypophysis; su, suspensor. Scale bars: 20 µm.
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