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Fig. 6. WOX5, PRS/WOX3 and WOX1 mRNA localization (brown
staining) during embryonic development. (A-L) Longitudinal median sections of
developing wild-type seeds are shown, if not indicated otherwise. White
asterisks indicate brown color of the seed coat independent of the staining
reaction. (A-F) In situ hybridization with a WOX5 antisense probe.
(A) No expression is detected in 16-cell embryos. (B) In early globular
embryos, expression is detected exclusively in the hypophysis. (C) After the
division of the hypophyseal cell, WOX5 expression becomes restricted
to the upper daughter, the lens shaped cell (lsc), and is not detectable in
the lower daughter, the columella precursor (cp). (D,E) In heart stage (D) and
bent cotyledon stage (E) embryos, WOX5 mRNA is detected in the
derivatives of the lens shaped cell, the developing quiescent center of the
embryonic root (arrows). (F) Cross section through cotyledon primordia of a
torpedo stage embryo (plane of section shown in J). WOX5 expression
is detected in patches at about the position where lateral veins will form
(arrow). (G-I) In situ hybridization with a PRS/WOX3 antisense probe.
(G,H) Early heart stage embryos. (G) Expression is detected in a subset of
cotyledonary cells (arrow). (H) Section through a cotyledon as indicated by a
line in G). PRS/WOX3 mRNA is detected in two lateral stripes. (I) A
torpedo stage embryo. PRS/WOX3 mRNA is restricted to a single cell at
the tip of the cotyledon (arrow). (J-L) In situ hybridization with a
WOX1 antisense probe. (J) A heart stage embryo. WOX1 mRNA is
detected in the provascular tissue of the cotyledons, but not in the
vasculature of the hypocotyl (arrow). (K) Cross section through a torpedo
stage cotyledon as indicated by the line in J. WOX1 expression is
detected in a stripe across the cotyledon. (L) Bent cotyledon stage.
WOX1 signal is weaker, but still detectable in the provascular tissue
of the cotyledons (arrow). c, cotyledon, cp, columella primordium; hy,
hypophysis; lsc, lens shaped cell; su, suspensor. Scale bars: 20 µm.
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