|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. wox2 embryos show specific defects in early development. (A) The
gray bar indicates the WOX2 genomic DNA fragment used in rescue
experiments. Above it, exon 1 and exon 2 have been enlarged to illustrate the
positions of insertions within WOX2. (B-D) Wild-type embryos display
a regular cell division pattern. (B) An 8-cell embryo. (C) An early globular
stage embryo. (D) A mid-globular stage embryo. (E-G) By contrast, about 30-50%
of wox2-1 embryos display defective apical development. (E) A
wox2-1 embryo at the same stage as the one shown in B, where the left
cell failed to divide (arrow). (F) A wox2-1 embryo at the same stage
as the ones shown in C, displaying aberrant oblique cell divisions in the
apical embryo domain (arrow). (G) A wox2-1 mid globular stage embryo.
A protoderm has formed despite aberrant oblique cell divisions in the apical
embryo domain (arrow). (H-J) About 15-30% of wox2-2 embryos have
similar defects to those of wox2-1 embryos. (H) 2-cell embryo,
showing a horizontal division (arrow) instead of a typical vertical one. (I)
Early globular stage embryo, displaying an oblique cell division (arrow). (J)
Mid stage embryo, displaying a misplaced tangential cell division (arrow)
(B-G) DIC microscopy. Scale bars: 20 µm.
|
