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Fig. 2. Sequence of the XMLC2 promoter. (A) The XMLC2 promoter contains GATA-,
MEF2- and CArG-binding motifs (shown in red, green and blue, respectively),
which are potential binding sites for GATA, MEF2 and SRF transcription
factors. An additional proximal motif (purple) combines a YY1 site with a weak
SRF-binding site. The proximal 700 nucleotides of promoter sequence are shown,
with the TATA box (bold) and 19 nucleotides of exon1 (lower case) indicated.
The minimal promoter fragment capable of supporting heart-specific expression
in transgenic studies (–123/–41) is highlighted. (B) Promoter
regions used in transgenesis experiments. For some constructs, the promoter
fragment was fused to a heterologous minimal promoter from the Xenopus
laevis cytoskeletal actin or Herpes simplex thymidine kinase genes
(orange box). All other constructs included to XMLC2a basal promoter and
transcription start site.
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