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Fig. 4. –123/–41 is sufficient for strong heart-specific expression. Strong, heart-specific expression is obtained with transgenes comprising the XMLC2 promoter region from –1558 to –48 (A) or –249 to –36 (B) fused to the minimal TK promoter. Two copies of an oligonucleotide comprising the sequence from –123 to –41 direct cardiac-specific expression of GFP from the minimal promoter of the cytoskeletal actin gene (C). By contrast, a similar chimeric promoter containing two copies of the promoter region –121 to –85 is insufficient for cardiac expression, giving only ectopic expression in the branchial arches (D). The tadpole heart region is indicated (white arrowhead). GFP activity in the eyes and hindbrain (C,D; black arrowheads) is from the -crystallin co-transgene. Intense endogenous fluorescence adjacent to the heart in B comes from the gall bladder (arrowed) and gut (g).

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