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Fig. 5. Proximal, high-affinity GATA sites are critical for expression of the XMLC2
promoter. Heart-specific expression is unaffected by targeted mutation of
individual GATA sites, such as GATA#2 (A). When both GATA#2 and GATA#3 sites
are inactivated, expression in the heart is maintained, but it is weak (B;
ventral view). Mutation of both GATA#1 and GATA#2 abolishes activity of the
promoter (C). Note that transgenesis in this case is confirmed by expression
of the co-transgene gamma-crystallin in the eye and hindbrain. (D) GATA#1 and
GATA#2 have greater affinity for GATA4 than the more distal GATA#3. S,
specific competitor (unlabelled probe); N, non-specific competitor (both used
at 100x molar excess). (E) Competition assay, using 20, 50 and
100x molar excess of competitors confirms this result.
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