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Fig. 3. Expression of neuropilin 2 and trochlear nerve trajectory. (A-C)
neuropilin 2 expression at stage 14 (A), stage 16 (B) and stage 18
(C). The position of the MHB is indicated by an arrowhead. (D,E) Staining of
the same specimen for neuropilin 2 (D, dark blue) and neurofilament
(E, magenta) at stage 20 during the dorsal decussation of the trochlear axons.
The decussation point is indicated by an arrow. (F) neuropilin 2
expression (dark blue) on the transversal section. Neuroepithelium layer is
indicated by double-headed arrows. (G) Merged image of neuropilin 2
expression in dorsal isthmus with neurofilament staining (G, magenta) in
higher magnification. (H,I) Flat-mount specimen after double staining with
anti-ISL1 (H,I, green) and anti NF-M antibody (I, magenta) during the dorsal
projection of trochlear axons at stage 19. (J-L) Staining of flat-mount
specimen for neuropilin 2 (J, dark blue) and neurofilament (K,
magenta) at st19. (L) Negative image of J was merged with K. Trochlear nucleus
along the rostrocaudal axis is indicated by double-headed arrows.
neuropilin 2 is expressed in the neuroepithelium of the dorsal
isthmus, in addition to the oculomotor and rostral trochlear nucleus. III,
oculomotor nucleus; IV, trochlear nucleus; dI, dorsal isthmus; ne,
neuroepithelium layer; Mid, midbrain; Hind, hindbrain. Scale bars: 200 µm
for A-E,H-L; 50 µm for F,G.
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