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Fig. 4. Expression of Sema3F and trochlear nerve trajectory. (A-G)
Expression of Sema3F (dark blue) in chick embryos at stage 10-22.
Sema3F expression is detected at the caudal border of the midbrain
along the MHB. (H-J) Expression of Sema3F (dark blue), Fgf8
(red), Otx2 (red), Gbx2 (dark blue) at stage 18.
Sema3F expression is rostral to the molecular boundary of the
Otx2 and Gbx2 expression. (K,L) Staining of same specimen
for Sema3F (K, dark blue) and neurofilament (L, magenta) during the
dorsal projection of trochlear axons at stage 19. (M) Image of K was merged
with (L). Expression of Sema3F was prominent in caudal margin of the
midbrain ventral to the lateral longitudinal fasciculus, and also in the
rhombic lip. Position of Sema3F expression along the MHB is indicated
with arrowheads (A-F,H,I). ov, otic vesicle; r3, rhombomere 3; r5, rhombomere
5; Mid, midbrain; Hind, hindbrain. Scale bars: 200 µm in A-D,K-M; 500 µm
in E-J.
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