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Fig. 6. Misexpression of neuropilin 2 dramatically alters trochlear nerve
trajectory. (A-C) Caudal view of neuropilin 2-transfected midbrain 2 days
after electroporation. Neurofilament (A, magenta); GFP (B, green); merge (C).
The broken line indicates the midline dissecting the control (untransfected)
and experimental (transfected) sides. (D-E) Flat-mount view of MHB from the
same embryo showing control (D) and experimental sides (E). (F) The GFP image
(green) was merged with E. An arrow indicates the dorsal decussation point.
(G,H) Confocal images of D,E at higher magnification. (I) Schematic drawing of
the result of misexpression. Trochlear axons (magenta) project dorsally from
the nucleus (IV; blue) but deflect rostrally at the point where the
transfected domain is encountered (thin arrow), cross the MHB and invade the
ipsilateral tectum. GFP-positive transfected domain is indicated (green). The
trochlear axons from the contralateral nucleus are seen (purple). A broad
arrow indicates the dorsal decussation point. A broken line marks the boundary
between alar and basal plate. (J,K) Trochlear axon trajectory of normal (J)
and experimental (K) embryos revealed by DiI labeling of trochlear nucleus. An
arrow indicates the relative position of the dorsal decussation point. The
images of E and F, H, K were reversed horizontally to compare with D, G, J,
respectively. Mid, midbrain; Hind, hindbrain; tec, tectum; cont, control side;
exp, experimental side; normal, normal embryo; teg, tegmentum; IV, trochlear
nucleus. Scale bars: 200 µm in A-H,J,K.
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