spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Clonal analysis of the effect of VEGF during primitive erythropoiesis. (A) VEGF increases the number of EryP per single EB. On day 7 of culture either with or without VEGF (5 ng ml–1), 25 EBs were picked at random and assayed individually into hematopoiesis. Histograms represent the frequency of EBs giving rise to the indicated number of EryP. (B) Gene-expression analysis of day-7 EBs treated with VEGF. Individual EBs (10) were dissociated as above and analyzed for hematopoietic-marker expression. No reverse transcriptase (RT) served as a control for genomic DNA contamination. Membranes were hybridized sequentially with the probes as shown. L32 is a loading control. (C) Plots illustrate the level of gene expression in a single EB compared to L32. Horizontal bars represent median values. *P<0.05 compared to untreated cells.





Right arrow Return to article