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Fig. 8. Hh requires HSPGs in order to move. (A,D,G) Hh distribution; (B,E,H) merged
images of Hh distribution (purple), with cells of the imaginal disc marked
uniformly with GFP (green). The clone of cells mutant for EXT originated from
a single cell lacking EXT activity, created by mitotic recombination and
marked by the absence of GFP. Thus, cells homozygous for the EXT mutant are
marked by an absence of GFP and cells heterozygous for the EXT mutant are pale
green. The staining intensity of Hh in the selected area (white boxes in B and
E) was integrated along AP axis, plotted using NIH Image software and
presented schematically (C,F). (A-C) Hh protein synthesized in the posterior
compartment appears to flow into the anterior compartment, with a moderate
concentration gradient starting from the middle of the posterior compartment.
(D-F) Hh accumulates abnormally in the posterior compartment when the EXT
mutant clone is in the anterior compartment along the AP boundary. The A/P
boundary is depicted with blue lines (A,D). (G,H) When the EXT mutant clone is
produced in the posterior compartment, Hh accumulation is reduced.
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