DEV00976 Supplemental Figures

Files in this Data Supplement:

• Figure 1 -

  Fig. S1. Activated Moesin immunolocalizes to photoreceptor apical membrane throughout development. Confocal micrographs show F-actin staining (red) and p-Moesin immunolocalized (green). Activated Moesin localizes to the apical membrane of the photoreceptor cell during the earliest stages of pupal development and is later restricted to the rhabdomere membrane, distinct from the stalk membrane (compare 35% pd with 47% pd). As the microvilli elongate (compare 71% pd with 100% pd), p-Moesin concentrates at rhabdomere base. Moesin is found in places of cell-cell contact between photoreceptors and pigment cells (arrowheads in 47% pd) and along the cone cell processes in between R1 and R2, R3 and R4, R5 and R6 (arrow in 100% pd), and R7 and R1. Confocal fields at 47% pd and 100% pd span the equator and show enantiomorphic ommatidia. Scale bar: 10 mm.

• Figure 2 -

  Fig. S2. Moe^G0323 flies express detectable levels of Moesin protein. Western analysis shows a reduced amount of Moesin in homozygous Moe^G0323 flies, a previously considered Moesin null allele, compared with wild type (w1118). Total protein was extracted from equal numbers of 1-day post-eclosion adult female fly heads and immunoblotted for Moesin.